Midkine Antikörper

Produktnummer ABIN116441

Der Kaninchen Polyklonal anti-Midkine Antikörper wird verwendet zum Nachweis von Midkine in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für Midkine Antikörper (ABIN116441)

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Midkine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Midkine Antikörper

Aufreinigung: Affinity chromatography employing immobilized hMidkine matrix.

Immunogen: Highly pure (>98%) recombinant hMidkine

Anwendungsinformationen

Applikationshinweise: Neutralization. ELISA: To detect hMidkine by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: 0.5 X PBS pH 7.4 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Andere Bezeichnung: Midkine

Hintergrund: Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation, before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.Synonyms: Amphiregulin-associated protein, MDK, MK, MK1, Midgestation and kidney protein, NEGF2, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

Gen-ID: 4192

NCBI Accession: NP_001012333

UniProt: P21741

Pathways: RTK Signalweg, M Phase, Skeletal Muscle Fiber Development