TNFSF14 Antikörper

Produktnummer ABIN116433

Dieses Anti-TNFSF14-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von TNFSF14 in WB und EIA. Geeignet für Human.

Kurzübersicht für TNFSF14 Antikörper (ABIN116433)

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNFSF14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-TNFSF14 Antikörper

Aufreinigung: Affinity chromatography employing immobilized hLIGHT matrix.

Immunogen: Highly pure (>98%) recombinant hLIGHT

Anwendungsinformationen

Applikationshinweise: Neutralization. ELISA: To detect hLIGHT by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hLIGHT. Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLIGHT is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: 0.5 X PBS pH 7.4 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu TNFSF14

Target: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Andere Bezeichnung: CD258 / LIGHT

Hintergrund: LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors, the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).Synonyms: HVEM-L, HVEML, Herpesvirus entry mediator-ligand, TNFSF14, Tumor necrosis factor ligand superfamily member 14

Gen-ID: 8740

NCBI Accession: NP_003798

UniProt: O43557

Pathways: Cancer Immune Checkpoints