Der Biotin-konjugierte Kaninchen Polyklonal anti-VEGFA Antikörper (ABIN116331) detektiert spezifisch VEGFA in WB und EIA.
Dieser Antikörper reagiert spezifisch mit Proben aus Maus.
VEGFA
Reaktivität: Human
WB, ELISA, Neut
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: To detect mVEGF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Target
VEGFA
(Vascular Endothelial Growth Factor A (VEGFA))