TGFA Antikörper

Produktnummer ABIN116239

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch TGFA in WB, IHC (p), EIA und Func. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für TGFA Antikörper (ABIN116239)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TGFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-TGFA Antikörper

Spezifität: This antibody reacts with Transforming Growth Factor alpha.

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hTGF-alpha

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTGF-α (0.5 ng/mL), a concentration of 2.5-2.7 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTGF-α by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTGF-α. (Sandwich): To detect hTGF-α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TGF-α (PP1070B1 or PP1070B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTGF-α. Western Blot: To detect hTGF-α by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTGF-α is 1.5- 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGF-alpha (TGFA)

Hintergrund: Synonyms: EGF-like TGF, ETGF, TGF type 1, Transforming growth factor alpha

Gen-ID: 7039

NCBI Accession: NP_001093161

UniProt: P01135

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway