KIT Ligand Antikörper
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- Target Alle KIT Ligand (KITLG) Antikörper anzeigen
- KIT Ligand (KITLG)
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser KIT Ligand Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
- Spezifität
- Human Stem Cell Factor (SCF).
- Aufreinigung
- Affinity chromatography.
- Immunogen
- Highly pure (>98%) recombinant hSCF (anti-hSCF).
- Top Product
- Discover our top product KITLG Primärantikörper
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- Applikationshinweise
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhSCF (10 ng/mL), a concentration of 8 - 12 ng/mL of this antibody is required. ELISA: To detect hSCF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: 1 μg/mL is the recommended starting dilution for frozen andparaffin sections. For paraffin sections antigen retrieval techniques may be necesserary toimprove staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Konservierungsmittel
- Without preservative
- Handhabung
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Target
- KIT Ligand (KITLG)
- Andere Bezeichnung
- KITLG / SCF (KITLG Produkte)
- Synonyme
- Xkl-1 antikoerper, Xsl antikoerper, Xsl-1 antikoerper, Xsl-2 antikoerper, steel antikoerper, KITLG antikoerper, SCF antikoerper, kitl antikoerper, kl-1 antikoerper, mgf antikoerper, scf antikoerper, FPH2 antikoerper, KL-1 antikoerper, Kitl antikoerper, MGF antikoerper, SF antikoerper, SHEP7 antikoerper, Clo antikoerper, Con antikoerper, Gb antikoerper, Kitlg antikoerper, Mgf antikoerper, SLF antikoerper, Sl antikoerper, blz antikoerper, contrasted antikoerper, 710-712 antikoerper, CSF antikoerper, KITL antikoerper, KIT ligand L homeolog antikoerper, KIT ligand antikoerper, kit ligand antikoerper, kitlg.L antikoerper, KITLG antikoerper, kitlg antikoerper, Kitl antikoerper, Kitlg antikoerper
- Hintergrund
- Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand
- Gen-ID
- 4254
- NCBI Accession
- NP_000890
- UniProt
- P21583
- Pathways
- RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung
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