KIT Ligand Antikörper

Produktnummer ABIN116225

Der Kaninchen Polyklonal Anti-KIT Ligand-Antikörper wurde für WB, EIA, IHC (p) und Func validiert. Er ist geeignet, KIT Ligand in Proben von Human zu detektieren.

Kurzübersicht für KIT Ligand Antikörper (ABIN116225)

Target: KIT Ligand (KITLG)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIT Ligand Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Produktdetails anti-KIT Ligand Antikörper

Spezifität: Human Stem Cell Factor (SCF).

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hSCF (anti-hSCF).

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhSCF (10 ng/mL), a concentration of 8 - 12 ng/mL of this antibody is required. ELISA: To detect hSCF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: 1 μg/mL is the recommended starting dilution for frozen andparaffin sections. For paraffin sections antigen retrieval techniques may be necesserary toimprove staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: KITLG / SCF

Hintergrund: Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

Gen-ID: 4254

NCBI Accession: NP_000890

UniProt: P21583

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung