PDGFB Antikörper (Biotin)

Produktnummer ABIN116209

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PDGFB in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PDGFB Antikörper (Biotin) (ABIN116209)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDGFB Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-PDGFB Antikörper (Biotin)

Spezifität: Recognizes Human Platelet Derived Growth Factor BB (hPDGF-BB)

Aufreinigung: Affinity Chromatography.

Immunogen: Highly pure (>98%) Recombinant Human PDGF-B.

Anwendungsinformationen

Applikationshinweise: Direct ELISA: To detect Human PDGF-BB by direct ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of approx. 1.0 μg/mL. This Biotinylatedantibody allows the detection of at least 0.2 ng/well of recombinant hPDGF-BB. Sandwich ELISA: To detect Human PDGF-BB by Sandwich ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of 0.25-1.0 μg/mL. This Biotinylatedantibody, in conjunction with anti-Human PDGF-BB antibody (PP1062P1 or PP1062P2) as aCapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu PDGFB

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Andere Bezeichnung: PDGFB

Hintergrund: Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.Synonyms: Becaplermin, PDGF B, PDGF beta, PDGF subunit B, PDGF-2, PDGF-B, PDGF2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta, Platelet-derived growth factor subunit B, SIS, c-Sis

Gen-ID: 5155

NCBI Accession: NP_002599

UniProt: P01127

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling