IL-5 Antikörper
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- Target Alle IL-5 (IL5) Antikörper anzeigen
- IL-5 (IL5) (Interleukin 5 (IL5))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser IL-5 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
- Spezifität
- This antibody reacts with Human Interleukin-5. Other species not tested.
- Aufreinigung
- Affinity Chromatography.
- Immunogen
- Highly pure (>98%) recombinant Human IL-5.
- Top Product
- Discover our top product IL5 Primärantikörper
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- Applikationshinweise
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-5(0.45 ng/mL), a concentration of 0.05-0.09 μg/mL of this antibody is required. ELISA: To detect hIL-5 by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hIL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-5 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Konservierungsmittel
- Without preservative
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Target
- IL-5 (IL5) (Interleukin 5 (IL5))
- Andere Bezeichnung
- Interleukin-5 / IL5 (IL5 Produkte)
- Synonyme
- EDF antikoerper, IL-5 antikoerper, TRF antikoerper, Il-5 antikoerper, IL5 antikoerper, interleukin 5 antikoerper, IL5 antikoerper, Il5 antikoerper
- Hintergrund
- Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF
- Gen-ID
- 3567
- NCBI Accession
- NP_000870
- UniProt
- P05113
- Pathways
- JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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