KIT Ligand Antikörper

Produktnummer ABIN115909

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch KIT Ligand in WB, EIA und Func. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für KIT Ligand Antikörper (ABIN115909)

Target: KIT Ligand (KITLG)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIT Ligand Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-KIT Ligand Antikörper

Spezifität: This antibody recognises Murine Stem Cell Factor.

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mSCF.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/mL),a concentration of 0.60-0.65 μg/mL of this antibody is required. ELISA: To detect mSCF by direct ELISA (using 100 l/well antibody solution) a concentration of atleast 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/wellof recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: KITLG / SCF

Hintergrund: Synonyms: KITL, Kit ligand, MGF, Mast cell growth factor, Stem cell factor, c-Kit ligand

Gen-ID: 17311

NCBI Accession: NP_038626

UniProt: P20826

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung