IL-3 Antikörper

Produktnummer ABIN115877

Der Kaninchen Polyklonal Anti-IL-3-Antikörper wurde für WB, EIA und Func validiert. Er ist geeignet, IL-3 in Proben von Maus zu detektieren.

Kurzübersicht für IL-3 Antikörper (ABIN115877)

Target: IL-3 (Interleukin 3 (IL-3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-IL-3 Antikörper

Spezifität: This antibody recognises Mouse Interleukin-3 (IL-3).

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mIL-3

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mIL-3 (0.5 ng/mL), aconcentration of 0.15-0.20 μg/mL of this antibody is required. ELISA: (Indirect): To detect mIL-3 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIL-3. (Sandwich): To detect mIL-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine IL-3 (PP010B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-3. Western Blot: To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: Interleukin-3 / IL3

Hintergrund: IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.Synonyms: Hematopoietic growth factor, IL-3, MCGF, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor

Gen-ID: 16187

NCBI Accession: NP_034686

UniProt: P01586

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie