MSH6 Antikörper

Produktnummer ABIN115272

Dieses Anti-MSH6-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MSH6 in WB, IHC (p), IP und IHC (fro). Geeignet für Human, Maus, Ratte und Hund.

Kurzübersicht für MSH6 Antikörper (ABIN115272)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 44

Produktdetails anti-MSH6 Antikörper

Spezifität: MSH6 is a heterodimer of MSH2 binds to DNA containing G/T mismatches. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop.
Cellular Localization: Nuclear.

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic human MSH6 peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Western Blot: 1/100-1/500. Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod (30 to 60 minutes at room temperature (requires high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining). Immunohistochemistry on Frozen Sections. Recommended Positive Control: Tonsil, colon carcinoma
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Andere Bezeichnung: MSH6

Hintergrund: An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time.Synonyms: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, MutS-alpha 160 kDa subunit, p160

Gen-ID: 2956

NCBI Accession: NP_000170

UniProt: P52701

Pathways: DNA Reparatur, Chromatin Binding, Production of Molecular Mediator of Immune Response