NEFL Antikörper

Produktnummer ABIN114492

Dieses Anti-NEFL-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NEFL in WB, IHC (p) und IHC (fro). Geeignet für Human, Ratte, Maus, Opossum, Cat und Kaninchen.

Kurzübersicht für NEFL Antikörper (ABIN114492)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reaktivität: Human, Ratte, Maus, Opossum, Cat, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 2F11

Produktdetails anti-NEFL Antikörper

Spezifität: 2F11 reacts exclusively with the phosphorylated isoform of the 70 kDa neurofilament protein. This antibody labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained.

Kreuzreaktivität (Details): Also reacts to: Neurofilament H

Aufreinigung: Purified

Immunogen: Human neurofilament preparation

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen and paraffin embedded sections: This antibody may bediluted to a titer of 1: 50-1: 200 in an ABC method. Immunoblotting: 1: 200 -1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Details zu NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Andere Bezeichnung: Neurofilament L (68kDa)

Hintergrund: Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament NF) triplet proteins (70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble. All three NF proteins can be detected by immunohistochemical methods at day 9 or 10 after gestation in the mouse embryo. Although IFPs of the neurofilament type are normally restricted to neurons, there are reports on their expression in non-neuronal cells as well. For example, in heart conduction myocytes NF proteins are expressed together with desmin. In tumorpathology ganglioneuroblastomas and some of the other neuroblastomas are strongly positive with the neurofilament antisera. Also, some neuro-endocrine malignancies may show NF positivity. In cell cultures of neural tissues the neurofilament antibodies can monitor in vitro differentiationSynonyms: 68 kDa neurofilament protein, NEFL, NF-L, NF68, Neurofilament L

Molekulargewicht: 68 kDa and 208 kDa (phosphorylated forms of these subunits).

Gen-ID: 4747

NCBI Accession: NP_006149

UniProt: P07196