Ly6g Antikörper (Biotin)

Produktnummer ABIN114270

Der Biotin-konjugierte Ratte Monoklonal anti-Ly6g Antikörper (Klon RB6-8C5) (ABIN114270) detektiert spezifisch Ly6g in FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für Ly6g Antikörper (Biotin) (ABIN114270)

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Ly6g Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Klon: RB6-8C5

Produktdetails anti-Ly6g Antikörper (Biotin)

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Immunogen: Mouse Granulocytes.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow cytometry. Immunohistochemistry on frozen and paraffin embedded tissues. Western blot. Complement-mediated depletion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of ABIN114273. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody FITC Goat anti-rat IgG (H+L) . 9. Incubate tubes at 4°C for 30-60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Mouse Strain: BALB/cCell

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Andere Bezeichnung: Ly6G / GR1 Neutrophil Marker

Hintergrund: Synonyms: Gr-1 Granulocyte marker

Gen-ID: 10090

UniProt: P35461