CD3 Antikörper (FITC)

Produktnummer ABIN114140

Der Hamster Monoklonal Anti-CD3-Antikörper wurde für FACS validiert. Er ist geeignet, CD3 in Proben von Maus zu detektieren.

Kurzübersicht für CD3 Antikörper (FITC) (ABIN114140)

Target: CD3

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: 500-A2

Produktdetails anti-CD3 Antikörper (FITC)

Spezifität: This anti-mouse CD3ε monoclonal antibody is specific for the ε chain of mouse CD31. Immobolized 500A2 mAb can be used to stimulate mouse T cells in vitro1. A combination of immobolized 500A2 mAb and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3.

Immunogen: C6VL-BS cell lysate

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow Cytometry (see Protocols). (Reportet to be useful in immunoprecipitation,immunofluorescent staining and immunohistochemistry on acetone fixed frozen sections. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % NaN3 and EIA grade BSA as stabilizing protien to bring total protein concentration to 4-5 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3

Hintergrund: In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transductionSynonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gen-ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway