APOA1 Antikörper (HRP)

Produktnummer ABIN114061

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch APOA1 in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für APOA1 Antikörper (HRP) (ABIN114061)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser APOA1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-APOA1 Antikörper (HRP)

Spezifität: This antibody recognizes Apo-A1 and HDL. It does not react with other plasma proteins, including albumin or immunoglobulin fractions. Does not cross react with apolipoprotein B, LDL and VLDL.

Aufreinigung: Affinity chromatography

Immunogen: Human Apolipoprotein A1

Anwendungsinformationen

Applikationshinweise: ELISA (1/5,000 - 1/20,000). Can be used in a two site assay with BM785 bound to themicrotitre plate and ABIN114061 for detection. Higher titers can be used when TMB is usedas the substrate). Western blot (1/100 -1/600).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (OD280)

Buffer: PBS containing 50 % glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Andere Bezeichnung: Apolipoprotein A I (APO AI)

Hintergrund: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.Synonyms: APOA1, Apo-AI, ApoA-I, ApoAI

Gen-ID: 335

NCBI Accession: NP_000030

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism