APOA1 Antikörper

Produktnummer ABIN112523

Der Maus Monoklonal Anti-APOA1-Antikörper wurde für EIA und ELISA (Capture) validiert. Er ist geeignet, APOA1 in Proben von Human zu detektieren.

Kurzübersicht für APOA1 Antikörper (ABIN112523)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser APOA1 Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA), ELISA (Capture)

Klon: G2

Produktdetails anti-APOA1 Antikörper

Spezifität: Reacts with both free Apolipoprotein A1 and HDL bearing Apo-A1. Does not cross-react with ApoE, ApoB or Albumin.Aff. Const.:Kd = 3nM (for Human Apolipoprotein A1)

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Native Human Apolipoprotein A1 from Human plasma.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA (1/2.500 - 1/10.000). This antibody is suitable for coating microtitre plates in asandwich ELISA using catalogue number ABIN114061 for detection. Western Blot (1/250 - 1/1000). Immunohistochemistry on frozen sections (1/20 - 1/80).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.

Buffer: 0.01 M Sodium Phosphate, 0.01 M Sodium Borate, 0.11 M Sodium Chloride with 1 % Mannitol and 1 % Dextran.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitve and should protected from light.

Details zu APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Andere Bezeichnung: Apolipoprotein A I (APO AI)

Hintergrund: Apolipoprotein A I promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids, an 18 amino acid signal peptide is removed co-translationally and a 6 amino acid propeptide is cleaved post-translationally. Apolipoprotein A I is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Defects in the Apolipoprotein A I gene are associated with HDL deficiency and Tangier disease. The therapeutic potential of apoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of apoA-I. The availability of recombinant normal apoA-I should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases.Synonyms: APOA1, Apo-AI, ApoA-I, ApoAI

Gen-ID: 335

NCBI Accession: NP_000030

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism