CDK4 Antikörper

Produktnummer ABIN112138

Dieser Maus Monoklonal Antikörper detektiert spezifisch CDK4 in WB, IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CDK4 Antikörper (ABIN112138)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: DCS-156

Produktdetails anti-CDK4 Antikörper

Spezifität: DCS-35 reacts specifically with CDK4 protein present predominantly in G1 phase of cell cycle. It does not cross-react with cyclins. In immunohisto-chemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) DCS-156 shows distinct nuclear staining, especially in tumor areas of enhanced proliferation.

Aufreinigung: Affinity Chromatohgraphy on Protein A.

Immunogen: Human recombinant full-length cdk4 polypeptide.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting (Western blotting). Suitable for Immunohistochemistry on Frozen and Paraffin Embedded Sections (improvedstaining after microwave treatment): 1/10Incubation Time: 1-4 h at RT or overnight at 2-8 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL distilled water.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Andere Bezeichnung: CDK4

Hintergrund: Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.Synonyms: Cell division protein kinase 4, Cyclin-dependent kinase 4, PSK-J3

Gen-ID: 1019

NCBI Accession: NP_000066

UniProt: P11802

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size