CD46 Antikörper

Produktnummer ABIN111918

Produktdetails anti-CD46 Antikörper

Target: CD46

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD46 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Affinity Chromatography

Immunogen: Human U-251 cells.

Klon: 13-42

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry (IHC), FACS, Western Blots and Functional Studies. Recommended Dilutions: IHC on Frozen sections: 1-2 μg/mL (1/200-1/400)IHC on Paraffin sections: 8 μg/mL (1/50), Proteinase K pretreatment for antigen retrieval isrecommended. Flow Cytometry: 5-10 μg/mL of neat antibody to label 1x10^6 cells. Immunoprecipitation: 5 μg/ 200 μL extract from 2x10^6 cells. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water 4. Block endogenous peroxidase 5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer's hemalum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Referenzen für anti-CD46 Antikörper (ABIN111918)

Fett, Hermann, Muether, Kirchhof, Fauser: "Immunohistochemical localization of complement regulatory proteins in the human retina." in: Histology and histopathology, Vol. 27, Issue 3, pp. 357-64, (2012) (PubMed).

Pedersen, Oster, Bundgaard, Höllsberg: "Induction of cell-cell fusion from without by human herpesvirus 6B." in: Journal of virology, Vol. 80, Issue 19, pp. 9916-20, (2006) (PubMed).

Details zu CD46

Target: CD46

Andere Bezeichnung: CD46 / MCP (CD46 Produkte)

Synonyme: AHUS2 antikoerper, MCP antikoerper, MIC10 antikoerper, TLX antikoerper, TRA2.10 antikoerper, Mcp antikoerper, CD46 antikoerper, CD46 molecule antikoerper, CD46 antigen, complement regulatory protein antikoerper, CD46 molecule, complement regulatory protein antikoerper, membrane cofactor protein-like antikoerper, CD46 antikoerper, Cd46 antikoerper, LOC100341359 antikoerper, LOC103789310 antikoerper

Hintergrund: CD46 is also known as Membrane Cofactor Protein (MCP). CD46 acts as a cofactor for factor I, a serine protease which is involved in the degradative cleavage of C3b and C4b. Structurally as well as functionally, it is a member of the regulator of complement activation proteins. CD46 is composed of an amino terminus of four short consensus repeating units (SCR), a Ser/Thr (ST)-rich domain, 13 amino acid residues with unknown function, a transmembrane region, and a cytoplasmic tail. There are many isoforms on human cells and in body fluids which are distinguishable by SDS-PAGE. Their heterogeneity stems from varying amounts of O-linked sugars secondary to alternative splicing of mRNA coding the ST-rich region.Synonyms: MIC10, Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen

Gen-ID: 9606

UniProt: P15529

Pathways: Regulation of Actin Filament Polymerization