S100A8 Antikörper (C-Term)

Produktnummer ABIN111892

Dieses Maus Monoklonal-Antikörper erkennt spezifisch S100A8 in FACS, IHC (p), EIA, IHC (fro) und DB. Er zeigt eine Reaktivität gegenüber Human, Ratte und Schwein.

Kurzübersicht für S100A8 Antikörper (C-Term) (ABIN111892)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reaktivität: Human, Ratte, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A8 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Klon: S13-67

Produktdetails anti-S100A8 Antikörper

Bindungsspezifität: C-Term

Spezifität: Clone S13.67 identifies MRP8: The antibody is useful in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. This clone also stains cells in rat spleen, indiating significant cross reactivity with the corresponding Rat MRP8. Antigen Distribution on Isolated Cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Antigen Distribution on Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages increasingly synthesise MRP-8 during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/MRP14.

Aufreinigung: Affinity Chromatography.

Immunogen: Cultured Human monocytes. Remarks: Antigen: MRP8. Epitope: Suspected in the N- or C-terminal Domain.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA. Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/100-1/200). Immunohistochemistry on Paraffin Sections: 4 μg/mL (1/50). Pre-treatment for antigenretrieval not required. Has been described to work in FACS and Dot blots. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore by adding 0.5 mL distilled water.

Konzentration: 0.2 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.05 % (v/v) Kathon CG as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: Do Not Use Sodium Azide as preservative.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Andere Bezeichnung: S100A8 / Calgranulin-A / MRP8

Hintergrund: MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A

Gen-ID: 9606

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine