S100A8 Antikörper (AA 25-89)

Produktnummer ABIN749273

Produktdetails anti-S100A8 Antikörper

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Bindungsspezifität: AA 25-89

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S100A8 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität: Human, Maus

Homologie: Rat

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse S100-A8

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-S100A8 Antikörper (ABIN749273)

Nguyen, Fentress, Qiu, Yun, Cox, Chawla: "Circadian gene Bmal1 regulates diurnal oscillations of Ly6C(hi) inflammatory monocytes." in: Science (New York, N.Y.), Vol. 341, Issue 6153, pp. 1483-8, (2013) (PubMed).

Details zu S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Andere Bezeichnung: S100A8 (S100A8 Produkte)

Synonyme: 60B8AG antikoerper, CAGA antikoerper, CFAG antikoerper, CGLA antikoerper, CP-10 antikoerper, L1Ag antikoerper, MA387 antikoerper, MIF antikoerper, MRP8 antikoerper, NIF antikoerper, P8 antikoerper, Mrp8 antikoerper, S100A8 antikoerper, 60B8Ag antikoerper, AI323541 antikoerper, B8Ag antikoerper, CFAg antikoerper, Caga antikoerper, p8 antikoerper, MRP-8 antikoerper, S100 calcium binding protein A8 antikoerper, S100 calcium binding protein A8 (calgranulin A) antikoerper, S100A8 antikoerper, S100a8 antikoerper

Hintergrund:

Synonyms: P8, MIF, NIF, CAGA, CFAG, CGLA, L1Ag, MRP8, CP-10, MA387, 60B8AG, Protein S100-A8, Calgranulin-A, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8, MRP-8, S100 calcium-binding protein A8, Urinary stone protein band A, S100A8

Background: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect, regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK.

Gen-ID: 6279

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine