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DYKDDDDK Tag Antikörper

Der Maus Anti-DYKDDDDK Tag-Antikörper wurde für WB, IP und IF validiert und wurde unabhängig validiert. Er ist geeignet, DYKDDDDK Tag in Proben von zu detektieren.
Produktnummer ABIN1112984

Kurzübersicht für DYKDDDDK Tag Antikörper (ABIN1112984)

Target

DYKDDDDK Tag

Reaktivität

Bitte anfragen

Wirt

  • 65
  • 54
  • 10
  • 8
  • 6
Maus

Konjugat

  • 50
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  • 1
  • 1
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  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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Dieser DYKDDDDK Tag Antikörper ist unkonjugiert

Applikation

  • 87
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  • 10
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Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
  • Sequenz

    DYKDDDDK

    Immunogen

    A synthetic peptide DYKDDDDK conjugated to KLH.

    Isotyp

    IgG1
  • Applikationshinweise

    WB (1:1000-10000), IP (1:100-500), IF (1:200-1000). Other applications have not been tested. The optimal dilutions should be determined by end user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Validierung #028889 (Immunofluorescence)
    'Independent Validation' Siegel
    by
    Reveal Biosciences
    No.
    #028889
    Datum
    07.11.2013
    Antigen
    Chargennummer
    L21/2013G
    Validierte Anwendung
    Immunofluorescence
    Positivkontrolle
    HEK293 cells transfected with DYKDDDDK Tag
    Negativkontrolle
    Untransfected HEK293 cells
    Bewertung
    Signal was detected in positive control samples but not in negative control samples.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antibody: DYKDDDDK Tag antibody
    • Catalog number: ABIN1112984
    • Lot number: L21/2013G
    Sekundärantikörper
    • Antibody: Goat anti-mouse IgG (H+L) IF488 conjugated antibody
    • Lot number: S6/2013S
    Full Protocol
    • HEK293 cells were grown directly on coverslips and transfected with a DYKDDDDK tagged protein.
    • Cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1 X PBS / 5% normal goat serum / 0.3% Triton X-100 for 20 min at RT.
    • Cells were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron
    • Microscopy Sciences, 25885-05) overnight at 4°C.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were incubated with secondary antibody diluted 1:150 in Universal Antibody Dilution Buffer
    • (Electron Microscopy Sciences, 25885-05) for 60 min at RT in dark.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with Fluoro-Gel II with DAPI (Electron Microscopy Sciences Cat. No: 17985-50).
    • Stained cells were imaged with an EIDAQ 100 fluorescent microscope.
    Anmerkungen
    None
  • Format

    Liquid

    Buffer

    PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

    Konservierungsmittel

    Sodium azide, Thimerosal (Merthiolate)

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8 °C for 1 month. Or aliquot and store at -20 °C or below for long term. Avoid repeated freeze and thaw cycles.
  • Target

    DYKDDDDK Tag

    Andere Bezeichnung

    Flag Tag

    Substanzklasse

    Tag

    Hintergrund

    DYKDDDDK-Tag, or DYKDDDDK octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. The peptide sequence of the DYKDDDDK-Tag is: N-DYKDDDDK-C (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein.
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