MAP2 Antikörper

Produktnummer ABIN111291

Dieses Anti-MAP2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MAP2 in WB, IF und IHC (fro). Geeignet für Human, Maus, Ratte, Rind (Kuh), Huhn, Wachtel und Xenopus laevis. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für MAP2 Antikörper (ABIN111291)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Wachtel, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: AP20

Produktdetails anti-MAP2 Antikörper

Spezifität: Thisantibody recognizes (Mr300 kDa) MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from Human, Rat, Mouse, Xenopus, quail and Chicken brains. BM2472 reacts with the High Molecular Weight forms (2a & 2b) of MAP-2 but not with the Low Molecular Weight (70 kDa) form (2c). It reacts with dendrites and cell bodies of neurons.

Aufreinigung: Protein G Chromatography.

Immunogen: Bovine brain Microtubule Associated Protein 2.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 1/500. Immunofluorescence: 1/500. Western Blotting: 1-2 μg/mL for 2hrs at RT. Immunohistology (Formalin/Paraffin): 1-2 μg/mL for 30 min at RT. Staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL (OD280)

Buffer: 0.01 M phosphate buffered saline (PBS) pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Aazide as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Referenzen für anti-MAP2 Antikörper (ABIN111291)

Pan, Tibbe, Harms, Reißner, Becker, Dingmann, Mirzaa, Kattentidt-Mouravieva, Shoukier, Aggarwal, Missler, Kutsche, Kreienkamp: "Missense mutations in CASK, coding for the calcium-/calmodulin-dependent serine protein kinase, interfere with neurexin binding and neurexin-induced oligomerization." in: Journal of neurochemistry, Vol. 157, Issue 4, pp. 1331-1350, (2021) (PubMed).

Details zu MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Andere Bezeichnung: MAP-2

Hintergrund: MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2, two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

Gen-ID: 4133

NCBI Accession: NP_002365

UniProt: P11137