CD35 Antikörper

Produktnummer ABIN111245

Der Maus Monoklonal Anti-CD35-Antikörper wurde für FACS, IHC (p), IHC (fro) und Func validiert. Er ist geeignet, CD35 in Proben von Human zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für CD35 Antikörper (ABIN111245)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD35 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Klon: J3D3

Produktdetails anti-CD35 Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Immunogen: Purified C3b receptor from Human red blood cells.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Mechanism of degradation of C3b and Analysis of Lymphoid and Myeloid differentiation: Clone J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3bdependent rosette formation with Lymphocytes. Fluorescence Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Immunohistochemistry: 1/25-1/50. Suitable on Frozen sections or cell smears, and onBouin's fixed, Paraffin-embedded tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL of distilled water.

Konzentration: 0.2 mg/mL (prior to lyophilization)

Buffer: PBS containing 1 mg/mL BSA as stabilizer and no preservative.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.

Referenzen für anti-CD35 Antikörper (ABIN111245)

Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, (2012) (PubMed).

Molnar: "Glycoproteins of Ehrlich ascites carcinoma cells. Incorporation of [14C]glucosamine and [14C]sialic acid into membrane proteins." in: Biochemistry, Vol. 6, Issue 10, pp. 3064-76 (PubMed).

Details zu CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Andere Bezeichnung: CD35 / C3BR

Hintergrund: This gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.Synonyms: C3b/C4b receptor, CR1, Complement receptor type 1

Gen-ID: 9606

UniProt: P17927

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity