CD15 Antikörper (FITC)

Produktnummer ABIN1112063

Produktdetails anti-CD15 Antikörper (FITC)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD15 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktmerkmale: Monoclonal Mouse Anti-Human CD15 FITC, is recommended for use in flow cytometry for identification of neutrophils, monoblastoid precursor cells of the myeloid lineage, eosinophils, and some monocytes, but not on basophils and lymphocytes.

Klon: MCS-1

Isotyp: IgG3

Anwendungsinformationen

Applikationshinweise: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Kommentare:

Fluorescein isothiocyanate (Molecular Probes).

Probennahme: 1. Transfer 100 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD15 FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing Stabilizing Solution, PBS pH 7,4 – 0,2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Lagerung: 4 °C

Referenzen für anti-CD15 Antikörper (FITC) (ABIN1112063)

Marin, Artal, Martin, Carrizo, Andres, Martin-Burriel, Bolea, Sanz, Varona, Godino, Gallego, Garcia-Erce, Villar, Gil, Forner, Cubero, Ros: "Epigenetics modifications and Subclinical Atherosclerosis in Obstructive Sleep Apnea: The EPIOSA study." in: BMC pulmonary medicine, Vol. 14, pp. 114, (2015) (PubMed).

Details zu CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Andere Bezeichnung: CD15 (FUT4 Produkte)

Synonyme: AI451562 antikoerper, CD15 antikoerper, FAL antikoerper, FucT-IV antikoerper, LeX antikoerper, SSEA-1 antikoerper, Ssea1 antikoerper, ELFT antikoerper, FCT3A antikoerper, FUC-TIV antikoerper, FUTIV antikoerper, Fuct antikoerper, fucosyltransferase 4 antikoerper, Fut4 antikoerper, FUT4 antikoerper

Hintergrund: The CD15 antigen is the X-hapten on lacto-N-fucose pentaosyl lll, which is expressed on circulating granulocytes or tissue granulocytes as well as neutrophils and eosinophils. The CD15 antibody recognizes the carbohydrate structure 3-fucosyl-N-acetyl-lactosamine. It specifically labels neutrophils, eosinophils and monoblastoid precursor cells of the myeloid lineage.