Cytokeratin 18 Antikörper

Produktnummer ABIN111183

Dieses Anti-Cytokeratin 18-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cytokeratin 18 in WB, IHC (p) und IHC (fro). Geeignet für Human, Maus, Ratte, Hund, Schwein, Rind (Kuh), Hamster, Schaf, Zebrafisch (Danio rerio) und Forelle. Dieses Primary Antibody wurde in 13+ Publikationen zitiert.

Kurzübersicht für Cytokeratin 18 Antikörper (ABIN111183)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Human, Maus, Ratte, Hund, Schwein, Rind (Kuh), Hamster, Schaf, Zebrafisch (Danio rerio), Forelle

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: Ks18-04

Produktdetails anti-Cytokeratin 18 Antikörper

Spezifität: Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reactivities on cultured cell lines (tested so far): HeLa and MCF-7

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat, Bovine, Porcine, Canine, Hamster, Sheep, Fish (Trout) and Zebrafish

Aufreinigung: Protein A chromatography

Immunogen: Human keratin K18 from HeLa cytoskeletal preparation

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot. Immunohistochemistry (dilute 1: 20 with PBS): suitable for cytological material, frozen andparaffin-embedded tissue (for best performance, microwave treatment is recommended). Incubation time: 1h at 37C, extended with paraffin sections (overnight at 2-8C).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1.0 mL distilled water.

Buffer: PBS, pH 7.4, 0.09 % Sodium azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing). Dilute only prior to immediate use.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antiserum at -20 °C (aliquot contents to

Referenzen für anti-Cytokeratin 18 Antikörper (ABIN111183)

Zatloukal, Kufferath, Thueringer, Landegren, Zatloukal, Haybaeck: "Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies." in: PLoS ONE, Vol. 9, Issue 5, pp. e96690, (2014) (PubMed).

Trowe, Airik, Weiss, Farin, Foik, Bettenhausen, Schuster-Gossler, Taketo, Kispert: "Canonical Wnt signaling regulates smooth muscle precursor development in the mouse ureter." in: Development (Cambridge, England), Vol. 139, Issue 17, pp. 3099-108, (2012) (PubMed).

Schwartz, Barker, Long, Kaufman, McCracken, Allen: "Natural IgM mediates complement-dependent uptake of Francisella tularensis by human neutrophils via complement receptors 1 and 3 in nonimmune serum." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 3064-77, (2012) (PubMed).

Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).

Ikenouchi, Matsuda, Furuse, Tsukita: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, (2003) (PubMed).

Schaffeld, Knappe, Hunzinger, Markl: "cDNA sequences of the authentic keratins 8 and 18 in zebrafish." in: Differentiation; research in biological diversity, Vol. 71, Issue 1, pp. 73-82, (2003) (PubMed).

Stumptner, Fuchsbichler, Heid, Zatloukal, Denk: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, (2002) (PubMed).

Bantel, Ruck, Gregor, Schulze-Osthoff: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, (2001) (PubMed).

Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, (1995) (PubMed).

Leeuwenberg, Smeets, Neefjes, Shaffer, Cinek, Jeunhomme, Ahern, Buurman: "E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro." in: Immunology, Vol. 77, Issue 4, pp. 543-9, (1993) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, (1983) (PubMed).

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: Cytokeratin 18

Hintergrund: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

Gen-ID: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose