Cytokeratin 18 Antikörper (FITC)

Produktnummer ABIN111182

Produktdetails anti-Cytokeratin 18 Antikörper (FITC)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Human, Maus, Ratte, Hund, Schwein, Rind (Kuh), Hamster, Schaf, Forelle

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 18 Antikörper ist konjugiert mit FITC

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors Specifically Detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide Reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with HeLa and MCF-7 (cell lines tested so far).

Aufreinigung: Protein A Chromatography

Immunogen: Human keratin K18 from a HeLa cytoskeletal preparation

Klon: Ks18-04

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoflourescence. Cytological material. Flow Cytometry: 1/5-1/10. Immunohistochemistry of Frozen Sections. Immunohistochemistry of Paraffin Sections: 1/10. Working dilution: 1/10 for immunohistochemistry. Incubation Time: 1 h at 37 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-Cytokeratin 18 Antikörper (FITC) (ABIN111182)

Trowe, Airik, Weiss, Farin, Foik, Bettenhausen, Schuster-Gossler, Taketo, Kispert: "Canonical Wnt signaling regulates smooth muscle precursor development in the mouse ureter." in: Development (Cambridge, England), Vol. 139, Issue 17, pp. 3099-108, (2012) (PubMed).

Hantel, Lewrick, Schneider, Zwermann, Perren, Reincke, Süss, Beuschlein: "Anti insulin-like growth factor I receptor immunoliposomes: a single formulation combining two anticancer treatments with enhanced therapeutic efficiency." in: The Journal of clinical endocrinology and metabolism, Vol. 95, Issue 2, pp. 943-52, (2010) (PubMed).

Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).

Ikenouchi, Matsuda, Furuse, Tsukita: "Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1959-67, (2003) (PubMed).

Stumptner, Fuchsbichler, Heid, Zatloukal, Denk: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, (2002) (PubMed).

Bantel, Ruck, Gregor, Schulze-Osthoff: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, (2001) (PubMed).

Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, (1995) (PubMed).

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Lauerová, Kovarik, Bártek, Rejthar, Vojt?sek: "Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule." in: Hybridoma, Vol. 7, Issue 5, pp. 495-504, (1989) (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, (1983) (PubMed).

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: Cytokeratin 18 (KRT18 Produkte)

Hintergrund: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18

Gen-ID: 3875

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose