Nitrotyrosine Antikörper

Produktnummer ABIN1108460

Der Maus Monoklonal Anti-Nitrotyrosine-Antikörper wurde für WB, IHC (fro), IHC (p) und EIA validiert. Er ist geeignet, Nitrotyrosine in Proben von zu detektieren.

Kurzübersicht für Nitrotyrosine Antikörper (ABIN1108460)

Target: Nitrotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nitrotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: HM-11

Produktdetails anti-Nitrotyrosine Antikörper

Aufreinigung: Protein G

Immunogen: Nitrated KLH

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Handhabung: Keep Sterile, Do Not Add Sodium Azide or any Nitric containing preservative since this will affect the antibody!

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu Nitrotyrosine

Target: Nitrotyrosine

Substanzklasse: Chemical

Hintergrund: Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological? pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage.Synonyms: NO-Tyrosine, Nitro-Tyrosine