LY96 Antikörper

Produktnummer ABIN1108190

Der Maus Monoklonal Anti-LY96-Antikörper wurde für WB, IF, EIA und Func validiert. Er ist geeignet, LY96 in Proben von Human zu detektieren.

Kurzübersicht für LY96 Antikörper (ABIN1108190)

Target: LY96 (Lymphocyte Antigen 96 (LY96))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LY96 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Functional Studies (Func)

Klon: 4H1

Produktdetails anti-LY96 Antikörper

Spezifität: This antibody reacts with soluble MD-2 (sMD-2) and the sMD-2/Toll-like receptor 4 (TLR4, CD284) complex. It reacts with both the monomeric and the polymeric form of sMD-2.

Keine Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Protein G

Immunogen: Baculovirally expressed His-tagged human MD-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2 - 8 °C.

Details zu LY96

Target: LY96 (Lymphocyte Antigen 96 (LY96))

Andere Bezeichnung: MD-2

Hintergrund: TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomer and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells.Synonyms: ESOP-1, ESOP1, LY96, Lymphocyte antigen 96, MD2

Gen-ID: 23643

NCBI Accession: NP_001182726

UniProt: Q9Y6Y9

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades