GFP Antikörper

Produktnummer ABIN1107357

Dieses Anti-GFP-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GFP in WB, IF, EIA und IHC (p). Geeignet für Aequorea victoria.

Kurzübersicht für GFP Antikörper (ABIN1107357)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 4B10B2

Produktdetails anti-GFP Antikörper

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Purified

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10000.
Western Bloting: 1/500 - 1/2000.
Flow Cytometry: 1/200 - 1/400.
Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS, 0.03 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund: GFP (Green Fluorescence Protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.Synonyms: GFP-Tag, Green fluorescent protein

Molekulargewicht: 27 kDa