TGFA Antikörper

Produktnummer ABIN1104644

Der Kaninchen Polyklonal anti-TGFA Antikörper wird verwendet zum Nachweis von TGFA in Proben von Human. Er wurde validiert für IHC, WB, ELISA, IHC (p) und Neut.

Kurzübersicht für TGFA Antikörper (ABIN1104644)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TGFA Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Produktdetails anti-TGFA Antikörper

Spezifität: Human TGF-alpha

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TGF-alpha.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (2.5 - 3.7 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7423 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human TGF-alpha (0.5 ng/mL), a concentration of 2.5-3.7 μg/mL of this antibody is required. ELISA: To detect Human TGF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TGF-alpha (LS-C104603) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TGF-alpha. Western Blot: To detect Human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TGF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGFA / TGF Alpha

Hintergrund: Name/Gene ID: TGFA
Family: TGF beta

Synonyms: TGFA, Etgf, TFGA, TGF Alpha, Tgf type 1, TGF-alpha, Egf-like tgf

Gen-ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway