C3 Antikörper (AA 1580-1592)

Produktnummer ABIN1049512

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch C3 in ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für C3 Antikörper (AA 1580-1592) (ABIN1049512)

Target: C3 (Complement Component 3 (C3))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser C3 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-C3 Antikörper

Bindungsspezifität: AA 1580-1592

Verwendungszweck: complement C3 (aa1580-1592)

Sequenz: QQRTFISPIK CRE

Spezifität: This antibody is expected to recognize the C3 alpha chain, C3b alpha' chain and the C3c alpha' chain fragment 2.

Homologie: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence QQRTFISPIKCRE, from the C Terminus of the protein sequence according to NP_000055.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

DS WB Results: Preliminary experiments gave an approx 125 kDa band in Human Liver lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104 kDa according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125 kDa band was successfully blocked by incubation with the immunizing peptide.

DS IHC Results: Paraffin embedded Human Placenta and Testis. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu C3

Target: C3 (Complement Component 3 (C3))

Andere Bezeichnung: Complement Component 3

Hintergrund: C3, complement component 3, AHUS5, ARMD9, ASP, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, OTTHUMP00000197086, acylation-stimulating protein cleavage product, complement C3, complement component C3

Gen-ID: 718

NCBI Accession: NP_000055

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling