NCKAP1 Antikörper (Internal Region)

Produktnummer ABIN1049510

Der Ziege Polyklonal Anti-NCKAP1-Antikörper wurde für ELISA, IF und FACS validiert. Er ist geeignet, NCKAP1 in Proben von Human zu detektieren.

Kurzübersicht für NCKAP1 Antikörper (Internal Region) (ABIN1049510)

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NCKAP1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-NCKAP1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: NCKAP1

Sequenz: TPREYLTSHL EIR

Spezifität: This antibody is expected to recognize both reported isoforms (NP_038464.1, NP_995314.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TPREYLTSHLEIR, from the of the protein sequence according to NP_038464.1, NP_995314.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Preliminary experiments gave an approx 100 kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5 μg/mL antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calcu

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NCKAP1

Target: NCKAP1 (NCK Associated Protein 1 (NCKAP1))

Andere Bezeichnung: NCKAP1

Hintergrund: NCKAP1, NCK-associated protein 1, FLJ11291, HEM2, KIAA0587, MGC8981, NAP1, NAP125, NAP 1, OTTHUMP00000163460, OTTHUMP00000163461, membrane-associated protein HEM-2, nck-associated protein 1, p125Nap1

Gen-ID: 10787, 50884, 58823

NCBI Accession: NP_038464, NP_995314

Pathways: RTK Signalweg, Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis