LPAR3 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN1048536

Dieser Anti-LPAR3-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von LPAR3 in IHC und IHC (p). Geeignet für Human, Maus, Ratte, Affe, Fledermaus, Rind (Kuh), Hund, Pferd und Hamster.

Kurzübersicht für LPAR3 Antikörper (Cytoplasmic Domain) (ABIN1048536)

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Reaktivität: Human, Maus, Ratte, Affe, Fledermaus, Rind (Kuh), Hund, Pferd, Hamster

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LPAR3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LPAR3 Antikörper

Bindungsspezifität: Cytoplasmic Domain

Spezifität: Human EDG7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Horse, Opossum, Platypus (100%) Panda (94%) Pig, Turkey, Chicken (88%) Xenopus (81%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human EDG7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Horse, Opossum, Platypus (100%), Panda (94%), Pig, Turkey, Chicken (88%), Xenopus (81%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu LPAR3

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Andere Bezeichnung: LPAR3 / LPA3 / EDG7

Hintergrund: Name/Gene ID: LPAR3
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR

Synonyms: LPAR3, EDG7, Edg-7, LPA receptor 3, HOFNH30, LPA3, RP4-678I3, LP-A3, LPA receptor EDG7, LPA-3

Gen-ID: 23566

Pathways: Regulation of Cell Size