LPAR3 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886540

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch LPAR3 in WB. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für LPAR3 Antikörper (Extracellular, N-Term) (ABIN7886540)

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LPAR3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-LPAR3 Antikörper

Bindungsspezifität: AA 16-29, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to LPA Receptor 3 (EDG7)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 12,14 amino acid residues identical

Produktmerkmale: Anti-LPAR3 (EDG7) (extracellular) Antibody (ABIN7043314, ABIN7044570 and ABIN7044571) is a highly specific antibody directed against an epitope to the rat LPA3 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA3 receptor from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RSNTDTADEWTGTK, corresponding to amino acid residues 16-29 of rat LPA3 receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235904)

Blocking Peptide: (ABIN7235904)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu LPAR3

Target: LPAR3 (Lysophosphatidic Acid Receptor 3 (LPAR3))

Andere Bezeichnung: LPAR3

Hintergrund:

Synonyms: Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32

Description: Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptors have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family2. All six receptors are type I, rhodopsin-like G-protein coupled receptors (GPCRs) that differ in their tissue distribution and downstream signaling pathways3.GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.Expression of LPA3 receptor has been observed in human heart, testis, prostate, pancreas, lung, ovary, and brain5 and is most abundant in mouse testis, kidney, lung, small intestine, heart, stomach, spleen, brain, and thymus6.LPA3 receptor null mice are viable and grossly normal, but female nulls show a striking phenotype in the reproductive system7. However, despite the fact that LPA3 is expressed in the frontal cortex, hippocampus, and amygdala, no phenotypes related to LPA3 loss in the nervous system have been reported to date5.

Gen-ID: 66025

NCBI Accession: NM_012152

UniProt: Q8K5E0

Pathways: Regulation of Cell Size