PDCD4 Antikörper (pSer457)

Produktnummer ABIN1043728

Hersteller Produkt- Nr.: 200-301-b17

Produktdetails anti-PDCD4 Antikörper

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Bindungsspezifität: pSer457

Reaktivität: Human, Maus, Ratte, Arthropods

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PDCD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 200-301-b17

Hersteller: Rockland

Verwendungszweck: Pdcd4 phospho S457 Antibody

Kreuzreaktivität (Details): This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.

Produktmerkmale: Synonyms: mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody

Aufreinigung: Pdcd4 phospho S457 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Pdcd4 phospho S457 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.

Immunogen Type: Conjugated Peptide

Klon: 9G6

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1.25-2.5 μg/mL

Application Note: Anti-PDCD4 pS457 monoclonal antibody has been tested by ELISA and western blotting and is suitable for immunohistochemistry.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract.  This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Western Blot Dilution: 1:1,000-1:3,000

ELISA Dilution: 1:400,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Pdcd4 phospho S457 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-PDCD4 Antikörper (ABIN1043728)

Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4 (PDCD4 Produkte)

Hintergrund: Background: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun. Ideal for researchers involved in Cell Signaling, Cancer and Signal Transduction research.

Gen-ID: 27250, 21735596

UniProt: Q53EL6