VCAM1 Antikörper

Produktnummer ABIN1042491

Dieses Anti-VCAM1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von VCAM1 in FACS, IP und IHC (fro). Geeignet für Human. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für VCAM1 Antikörper (ABIN1042491)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VCAM1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 4B9

Produktdetails anti-VCAM1 Antikörper

Spezifität: The antibody binds exclusively to the cytokine-induced endothelial cell adhesion protein, vascular cell adhesion molecule-1 (VCAM-1).

Produktmerkmale: Tumour marker

Aufreinigung: Purified

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: 4B9 is suitable for immunoblotting, immunoprecipitation, immunocytochemistry and immuno-histochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.

Kommentare:

4B9 is a mouse IgG1 monoclonal antibody generated by immunization of Balb/c mice with human umbilical vein endothelium (HUVE) that were non-enzymatically harvested after stimulation for 24h with recombinant human (rh) TNF-alpha (10 ng/ml). Splenocytes were fused with the NS-1 nonsecretory murine myeloma line.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.09% sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-VCAM1 Antikörper (ABIN1042491)

Chuluyan, Osborn, Lobb, Issekutz: "Domains 1 and 4 of vascular cell adhesion molecule-1 (CD106) both support very late activation antigen-4 (CD49d/CD29)-dependent monocyte transendothelial migration." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 6, pp. 3135-4, (1995) (PubMed).

Pinola, Saksela, Tiisala, Renkonen: "Human NK cells expressing alpha 4 beta 1/beta 7 adhere to VCAM-1 without preactivation." in: Scandinavian journal of immunology, Vol. 39, Issue 2, pp. 131-6, (1994) (PubMed).

Bacchi, Marsh, Perkins, Carithers, McVicar, Hudkins, Benjamin, Harlan, Lobb, Alpers: "Expression of vascular cell adhesion molecule (VCAM-1) in liver and pancreas allograft rejection." in: The American journal of pathology, Vol. 142, Issue 2, pp. 579-91, (1993) (PubMed).

Ockenhouse, Tegoshi, Maeno, Benjamin, Ho, Kan, Thway, Win, Aikawa, Lobb: "Human vascular endothelial cell adhesion receptors for Plasmodium falciparum-infected erythrocytes: roles for endothelial leukocyte adhesion molecule 1 and vascular cell adhesion molecule 1." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 1183-9, (1992) (PubMed).

Carlos, Schwartz, Kovach, Yee, Rosa, Osborn, Chi-Rosso, Newman, Lobb, Rosso: "Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured human endothelial cells." in: Blood, Vol. 76, Issue 5, pp. 965-70, (1990) (PubMed).

Details zu VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Andere Bezeichnung: VCAM-1

Hintergrund: Vascular cell adhesion molecule-1 (VCAM-1) is induced on human umbilical vein endothelium (HUVE) by recombinant human tumor necrosis factor - alpha (rh TNF-alpha), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69-kDa core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kDa is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigration at sites of inflammation and immune reaction.

Pathways: Carbohydrate Homeostasis