HSP27 Antikörper

Produktnummer ABIN100223

Produktdetails anti-HSP27 Antikörper

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Sterilität: Sterile filtered

Immunogen: This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
Immunogentype:Recombinant

Klon: G3-1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting, immunoprecipitation, ELISA, immunohistochemistry and immunocytochemistry.  The antibody recognizes a 27 kDa band corresponding to hsp27 in cell lysates from breast carcinoma.    Both frozen sections and paraffin embedded material can be used for immunocytochemistry and immunohistochemistry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-HSP27 Antikörper (ABIN100223)

McGuire, Fuqua, Naylor, Helin-Davis, McGuire: "Chromosomal assignments of human 27-kDa heat shock protein gene family." in: Somatic cell and molecular genetics, Vol. 15, Issue 2, pp. 167-71, (1989) (PubMed).

Moretti-Rojas, Fuqua, Montgomery, McGuire: "A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells." in: Breast cancer research and treatment, Vol. 11, Issue 2, pp. 155-63, (1988) (PubMed).

Edwards, Adams, McGuire: "Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7)." in: Breast cancer research and treatment, Vol. 1, Issue 3, pp. 209-23, (1983) (PubMed).

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSP27 (HSPB1 Produkte)

Synonyme: CMT2F antikoerper, HMN2B antikoerper, HS.76067 antikoerper, HSP27 antikoerper, HSP28 antikoerper, Hsp25 antikoerper, SRP27 antikoerper, 27kDa antikoerper, Hsp27 antikoerper, cb153 antikoerper, cb660 antikoerper, hsp1 antikoerper, hsp25 antikoerper, hsp27 antikoerper, id:ibd2821 antikoerper, sb:cb660 antikoerper, zgc:103437 antikoerper, HSPB1 antikoerper, cmt2f antikoerper, hs.76067 antikoerper, hsp28 antikoerper, heat shock protein family B (small) member 1 antikoerper, heat shock protein 1 antikoerper, heat shock protein, alpha-crystallin-related, 1 antikoerper, heat shock protein family B (small) member 1 L homeolog antikoerper, HSPB1 antikoerper, Hspb1 antikoerper, hspb1 antikoerper, hspb1.L antikoerper

Hintergrund: Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in different cell types and tissues (this protein has also been referred to as the Estrogen-Regulated 24 kDa protein, hsp25 and hsp28).   Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and post-translational level.  In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change.  Human HSP27 shares greater than 50% homology with the low molecular weight Drosophila HSP and mammalian crystalline lens protein.  Because of the estrogen responsive nature of HSP27, the protein has been extensively
Synonyms: Heat shock protein beta-1 antibody, HspB1 antibody, Heat shock 27 kDa protein antibody, Stress-responsive protein 27 antibody, SRP27 antibody, Estrogen-regulated 24 kDa protein antibody, 28 kDa heat shock protein antibody

Gen-ID: 39078, 17647521

UniProt: P04792

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling