HSP27 Antikörper

Produktnummer ABIN100223

Hersteller Produkt- Nr.: 200-301-243

Produktdetails anti-HSP27 Antikörper

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 200-301-243

Hersteller: Rockland

Verwendungszweck: HSP27 Antibody

Kreuzreaktivität (Details): This protein A purified mouse monoclonal antibody reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control.

Produktmerkmale: Synonyms: mouse anti-HSP27 antibody, mouse anti-Heat shock protein beta-1 antibody, HspB1 antibody, Heat shock 27 kDa protein antibody, Stress-responsive protein 27 antibody, SRP27 antibody, Estrogen-regulated 24 kDa protein antibody, 28 kDa heat shock protein antibody

Aufreinigung: Protein A purified

Sterilität: Sterile filtered

Immunogen:

Immunogen: This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.

Immunogen Type: Recombinant Protein

Klon: G3-1

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Application Note: This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting and ELISA and is suitable in immunoprecipitation, immunohistochemistry, and immunocytochemistry.  The antibody recognizes a 27 kDa band corresponding to hsp27 in cell lysates from breast carcinoma.    Both frozen sections and paraffin embedded material can be used for immunocytochemistry and immunohistochemistry.

Western Blot Dilution: 1:500 - 1:1,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-HSP27 Antikörper (ABIN100223)

McGuire, Fuqua, Naylor, Helin-Davis, McGuire: "Chromosomal assignments of human 27-kDa heat shock protein gene family." in: Somatic cell and molecular genetics, Vol. 15, Issue 2, pp. 167-71, (1989) (PubMed).

Moretti-Rojas, Fuqua, Montgomery, McGuire: "A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells." in: Breast cancer research and treatment, Vol. 11, Issue 2, pp. 155-63, (1988) (PubMed).

Edwards, Adams, McGuire: "Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7)." in: Breast cancer research and treatment, Vol. 1, Issue 3, pp. 209-23, (1983) (PubMed).

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSPB1 (HSPB1 Produkte)

Hintergrund: Background: Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in different cell types and tissues (this protein has also been referred to as the Estrogen-Regulated 24 kDa protein, hsp25 and hsp28). Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and post-translational level. In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. HSP27 plays a major role in the increased thermal resistance acquired by cells after exposure to HSP inducers. The level of HSP27 phosphorylation is significantly elevated after exposure of cells to heat shock, sodium arsenite, IL-1 and TNF-a. MAPKAPK2 and MAPKAPK3 are both activated by these conditions and can phosphorylate HSP27 on serine residues Anti-HSP27 Antibody is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Cancer, Cellular Stress, and p38 Pathway research.

Gen-ID: 3315, 17647521

UniProt: P04792

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling