anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Antikörper

Human Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Interaktionspartner

1. it was demonstrated that high expression of neddylation components, neural precursor cell expressed, developmentally downregulated 8 (NEDD8) and NEDD8activating enzyme 1 (NAE1), were associated with poor survival of patients with hepatocellular carcinoma (HCC)

2. The newly identified CUBAN (Cullin binding domain associating with NEDD8) domain recognizes both ubiquitin and the ubiquitin-like NEDD8. After a slow conformational selection step, hydrophobic and then neutral and polar interactions take place, which drive the correct orientation of the CUBAN domain, leading to differences in the recognition scheme of NEDD8 and ubiquitin.

3. Impairment of NEDDylation by Ubc12 knockout enhances PCNA ubiquitination and promotes PCNA-poleta interaction, while up-regulation of NEDDylation by NEDD8 overexpression or NEDP1 deletion reduces the excessive accumulation of ubiquitinated PCNA. Moreover, Ubc12 knockout decreases cell sensitivity to H2O2-induced oxidative stress, but NEDP1 deletion aggravates this sensitivity

4. NEDD8 has important roles in regulating the progression of bladder cancer (BC) cells and was associated with poor prognosis of patients; hence, it may become a potential therapeutic target of BC.

5. Data suggest that ubiquitin (Ub) and neural precursor cell expressed developmentally down-regulated 8 (NEDD8) residues 4, 12, 14, and 72 serve as the molecular determinants for specific recognition by ubiquitin specific protease 2 protein (USP2).

6. Nedd8 binding to Smurf plays important roles in the regulation of cell migration and the BMP and TGFbeta signaling pathways.

7. NEDD8-mediated neddylation promotes stress granule assembly.

8. NEDD8 seems to inhibit the Src-mediated phosphorylation of caveolin-1 by modifying the structure of caveolin-1 protein, which blocks the migration of cancer cells. Although the neddylation process is currently regarded as an emerging target for cancer therapy, our results suggest the possibility that the inhibition of neddylation could facilitate cancer invasion or metastasis at least in some types of cancers.

9. The cellular effects of NEDD8 inhibition can be manipulated based on the p53 status.

10. Here we report that high expression of neddylation components, NEDD8 and NAE1, are associated with poor survival of Pancreatic ductal adenocarcinoma patients. Blockage of neddylation by MLN4924 significantly sensitizes pancreatic cancer cells to gemcitabine, reduced clonogenic survival, decreased invasion capacity, increased apoptosis and senescence

11. Downregulation of NEDD8 enhanced the susceptibility of nasopharyngeal carcinoma cells to cisplatin and radiation.

12. Furthermore, the authors characterized SENP8/DEN1 as the protease that counteracts Ubc12 auto-neddylation, and observed aberrant neddylation of Ubc12 and other NEDD8 conjugation pathway components in SENP8-deficient cells.

13. MKK7 undergoes neddylation in human breast cancer cells

14. complete blocking of CRLs at a higher inhibitor dose-induced cytotoxicity that was amplified by knockdown of CRL regulator Cand1.

15. In conclusion, the authors revealed that E3 ligase HDM2 promotes NEDD8 NEDDylation of HBx to enhance HBx stability and chromatin localization, which in turn favors HBx-dependent transcriptional regulation, cell proliferation, and hepatitis B virus-driven tumor growth.

16. CUL5 neddylation may allosterically tune polyubiquitin chain length and topology.

17. This study identifies MyD88 as a novel substrate of NEDD8, and demonstrate that MyD88 NEDDylation antagonizes its ubiquitination.

18. This study provides new knowledge of the CFTR biosynthetic pathway. It suggests that SYVN1 and FBXO2 represent two distinct multiprotein complexes that may degrade DeltaF508-CFTR in airway epithelia and identifies a new role for NEDD8 in regulating DeltaF508-CFTR ubiquitination.

19. Neddylation of Smurf1 activates its ubiquitin ligase activity and Smurf2 exerts Nedd8 ligase activity. This study provided new clues of Smurf2 activation regulation.

20. Nedd8(Q40E) cannot induce the same structural effect on Cul1-Rbx1 as wild-type Nedd8.

Mouse (Murine) Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Interaktionspartner

1. Downregulation of NEDD8 enhanced the susceptibility of nasopharyngeal carcinoma cells to cisplatin and radiation.

2. Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity.

3. neural precursor cell expressed developmentally downregulated protein 8 (Nedd8) modification of Cul-5 is required for its interaction with TRAF6.

4. Our findings provide mechanistic evidence of the effects of IAP antagonists in combination with NAE inhibitors, and demonstrate the potential of a new combination therapy for cancer.

5. a role of Nedd8 in regulating caspase-1 activation following inflammasome activation

6. Nedd8 and its associated E2 carrier protein (Ubc12) and E3-SCF ligase play a key role in regulating the expression and activity of ENaC in alveolar pneumocytes.

7. a new regulatory mechanism of RCAN1 function

8. c-Cbl conjugates neural precursor cell-expressed, developmentally downregulated 8 (NEDD8), a ubiquitin-like protein, to TbetaRII at Lys556 and Lys567.

9. The NEDD8 pathway appears to play an important role in CD4+ T-cell-mediated inflammatory responses

10. Nedd8 and AhR localize to overlapping areas in the heart and spinal ganglia, raising the possibility that this interaction may play a role in organogenesis

11. the analysis of expression pattern in the mouse embryo

12. NEDD8 and ubiquitin function in a redundant manner in controlling receptor endocytic pathways.

13. SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation

Fruit Fly (Drosophila melanogaster) Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) Interaktionspartner

1. the Cul1-based SCF complex requires Nedd8 modification for the degradation processes of Ci, Arm, and CycE in vivo

2. Genetic analyses indicate that a balance between neddylation and deneddylation maintained by DEN1 is crucial for animal viability.