TNFRSF1B Protein (AA 1-257) (Fc Tag)

Produktnummer ABIN925130

Dieses Recombinant TNFRSF1B-Protein wird in HEK-293T Cells produziert.

Kurzübersicht für TNFRSF1B Protein (AA 1-257) (Fc Tag) (ABIN925130)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293T Cells

Applikation: Apoptosis Blocking (AB)

Reinheit: > 95 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-257

Aufreinigungstag / Konjugat: Dieses TNFRSF1B Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Prepare a sterile stock solution of rhTNF-R2:Fc (1 mg/ml in PBS) by dissolving the entire preparation in 50 µl sterile H2O. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.

Spezifität: Highly specific for the detection of membrane- bound TNF-alpha.

Produktmerkmale: Host: The extracellular domain of human TNF- R2 (AA 1-257) is fused to the Fc portion of human IgG1.

Anwendungsinformationen

Applikationshinweise: Inhibition of TNF-mediated apoptosis: Exerts inhibitory activity at concentrations higher than 1000 ng/ml. TNF-R2:Fc prefentially interacts with the membrane-bound form of TNF-alpha. Concentrations of rhTNF-R2 required to block killing may vary depending on cell type studied.
Method: Murine WEHI 164 cells (50,000 cells in 100 µl RPMI medium containing 10% fetal calf serum) were plated in a 96 well plate. 100 pg/ml rhsTNF- were incubated with the indicated concentrations of rhTNFR2:Fc for 30 minutes at RT. Then the mixture was added to the WEHI 164 target cells and incubated for at least 12 hr at 37°C. Concentrations of rhsTNF-alpha required to kill cells may vary depending on cell type studied. Concentrations of rhTNFR2:Fc required to block killing may vary depending on cell type studied. Cell viability was determined using a MTT- based cell proliferation assay kit.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS

Lagerung: -20 °C

Antigendetails

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Andere Bezeichnung: TNF-R2

Hintergrund: TNF-Receptors (TNF-R) are implicated in inflammatory processes and are the prototype members of the TNF receptor family. There are two distinct TNF-R, i. e. TNF-R1 and TNF-R2, that interact with the ligand TNF-. Whereas both receptors activate NF-B and JNK, TNF-R1 also signals cell death. Activated TNF-R1 contains a death domain in its cytoplasmic region that recruits the adaptor proteins TRADD, RIP, FADD, and TRAF2. TNF-R2 recruits TRAF proteins. Whereas membrane-bound and processed, soluble TNF- bind to TNF-R1, only the membrane-bound form appears to interact with TNF-R2.

Molekulargewicht: 80 kDa (chimeric protein)

Pathways: NF-kappaB Signalweg, Apoptose, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway