PCNA Protein

Name: PCNA

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN667068

Produktbeschreibung

Produktmerkmale: PCNA, 1-261 aa, Human, Recombinant, E.coli

Format: Liquid

Beschreibung: PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E.coli and purified by conventional chromatography techniques. Synonyms: MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA,. NCBI no.: NP_002583

Molekulargewicht: 28 kDa (261 aa), confirmed by MALDI-TOF.

Anwendungen

Konzentration: 1 mg/ml (determined by Bradford assay)

Reinheit: > 95% by SDS - PAGE

Buffer: Liquid in 20mM Tris pH 7.5, 2mM EDTA, 20% glycerol

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

PCNA protein