Proliferating Cell Nuclear Antigen (PCNA) Protein

Name: Proliferating Cell Nuclear Antigen (PCNA)

Synonyme: MGC8367, PCNAR, Pcna/cyclin, pcna

Protein-Typ: Recombinant

Herkunft (Gen): Human

Produktnummer: ABIN622005

Produktbeschreibung

Produktmerkmale: Recombinant Human Proliferating Cell Nuclear Antigen (PCNA)

Swiss-Prot: AAX43157

Beschreibung: Proliferating Cell Nuclear Antigen (PCNA) is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta increasing the processivity of leading strand synthesis during DNA replication. Therefore, PCNA is important factor for both DNA synthesis and DNA repair. In response to DNA damage, PCNA protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Synonyms: Proliferating cell nuclear antigen, PCNA, DNA polymerase delta auxiliary protein, cyclin, proliferating cell nuclear antigen.

Molekulargewicht: ~29 kDa.

Anwendungen

Aufbereitung der Reagenzien: The gene encoding the full length of human PCNA protein was cloned and expressed in Escherichia coli. The recombinant PCNA protein was purified by proprietary chromatographic techniques.

Reinheit: >95%, determined by SDS-PAGE and stained with Commassie blue. (See image on the right)

Buffer: Fine white powder, lyophilized. Recombinant PCNA was lyophilized from a 0.2 µm filtered phosphate-buffered saline (PBS) with protein concentration at 0.8 mg/mL. It is recommended to briefly spin the vial prior to opening, bring the contents to the bottom, and reconstitute the lyophilized product with sterile 18 M omega cm deionized water or your desired buffer.

Lagerung: Lyophilized product is stable at room temperature for 3 weeks, it is recommended to be stored desiccated below -20°C in a manual defrost freezer. Upon reconstituted, the protein should be stored at 4°C for one week. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA) and store at -20 or -80°C. Please avoid repeated freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar