Signal Transducer and Activator of Transcription 1 (STAT1) Antikörper

Antigen: Signal Transducer and Activator of Transcription 1 (STAT1)

Synonyme: ISGF-3, STAT91, DKFZp686B04100, XStat1, STAT1, STAT4, isgf-3, stat91, MGC147452, DKFZp459D206

Klonalität: Monoklonal (SM1)

Wirt: Maus

Reaktivität: Human, Maus

Applikation: Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN94471

Produktbeschreibung

Weitere Bezeichnung: STAT1

Immunogen: STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).

Format: Purified

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: SM1

Beschreibung: STAT1 (signal transducer and activator of transcription 1) is a transcription factor that playsimportant roles in growth arrest, apoptosis promoting and tumour suppression. After ligationof cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 orJAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimersserve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is alsophosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation.However, the both modifications are important for its maximal transcriptional activity. On theother hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Spezifität: The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a91 kDa transcriptional factor involved in a variety of systems including antiviral responsesand interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

Anwendungen

Applikationshinweise: Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Positive tissue: lung macrophages

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Krebs, Transkriptionsfaktoren, Signaltransduktion, Rezeptoren, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Induction of phosphorylation of STAT1 (Western Blotting)