CD25 Antikörper (FITC)

Antigen: CD25

Klonalität: Monoklonal (MEM-181)

Wirt: Maus

Reaktivität: Human

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN94046

Produktbeschreibung

Immunogen: PHA-activated peripheral blood leucocytes

Isotyp: IgG1

Klon: MEM-181

Beschreibung: CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R).Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereasCD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cellactivation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cellsurface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a markerof T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells.Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograftrejection as well as the severity of graft versus host reaction, and radioimmunoconjugates ofanti-CD25 antibodies can be used against CD25 expressing lymphomas.

Spezifität: The antibody MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa typeI transmembrane glycoprotein expressed on activated B and T lymphocytes, activatedmonocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost onresting B and T lymphocytes.HLDA VI, WS Code NL N-L024

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Surface staining of human PBMC with anti-human CD25 (MEM-181) FITC. The mononuclear cells were isolated from human peripheral blood, devided in aliquots for duplicate analysis and stimulated with PHA for 2 days. Panel A, C – staining with the anti-human CD25 (MEM-181).Panel B, D – staining with the standard anti-CD25 monoclonal antibody.

Publikationen

Publikationen: Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Patten, Buggins, Richards et al.: "CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment." in: Blood, Vol. 111, Issue 10, pp. 5173-81, 2008 (PubMed).

Scottua, Soligo, Camperio et al.: "FOXP3 induced by CD28/B7 interaction regulates CD25 and anergic phenotype in human CD4+CD25- T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 2, pp. 1025-33, 2008 (PubMed).

Jursik, Prchal, Grillari-Voglauer et al.: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009 (PubMed).

Berges, Fuchs, Opelz et al.: "Helenalin suppresses essential immune functions of activated CD4+ T cells by multiple mechanisms." in: Molecular immunology, Vol. 46, Issue 15, pp. 2892-901, 2009 (PubMed).