Activating Transcription Factor 2 (ATF2) Antikörper

Antigen: Activating Transcription Factor 2 (ATF2)

Synonyme: HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Ratte (Rattus), Maus

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN669546

Produktbeschreibung

Weitere Bezeichnung: ATF2

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human ATF2 C-terminus

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Synonym: CREB 2, HB 16, Activating Transcription Factor 2, ATF 2, Atf-2, ATF2 protein, cAMP Response Element Binding Protein 2, cAMP response element binding protein CRE BP1, cAMP-dependent transcription factor ATF-2, cAMP-responsive element-binding protein 2, CRE BP1, CRE-BP, CREB 2, CREB2, CREBP1, Cyclic AMP dependent transcription factor ATF 2, Cyclic AMP-responsive element-binding protein 2, HB 16, HB16.

Molekulargewicht: 43-45kDa

Kommentare:

ATF2 (Activating Transcription Factor 2

CREBP

HB16

CREB2

TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation

and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis

seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents

inflammatory cytokines

and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.

Anwendungen

Applikationshinweise: WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Forschungsgebiet: Immunologie, Angeborene Immunität, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar