ATG5 Antikörper

Produktnummer ABIN650657

Es gibt 1 Publikation für dieses Produkt. Der Kaninchen Polyklonal anti-ATG5 Antikörper wird verwendet zum Nachweis von ATG5 in Proben von Human. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für ATG5 Antikörper (ABIN650657)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB12327

Produktdetails anti-ATG5 Antikörper

Aufreinigung: This antibody is purified through a protein A column, followed by dialysis against PBS.

Immunogen: This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human ATG5.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:200. WB: 1:1000. WB: 1:500. WB: 1:500. IHC-P: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-ATG5 Antikörper (ABIN650657)

Jaber, Dou, Chen, Catanzaro, Jiang, Ballou, Selinger, Ouyang, Lin, Zhang, Zong: "Class III PI3K Vps34 plays an essential role in autophagy and in heart and liver function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 6, pp. 2003-8, (2012) (PubMed).

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: APG5

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Molekulargewicht: 32447

Gen-ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie