IL1R2 Antikörper (Internal Region)

Produktnummer ABIN570867

Der Ziege Polyklonal anti-IL1R2 Antikörper (ABIN570867) detektiert spezifisch IL1R2 in WB, ELISA, FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für IL1R2 Antikörper (Internal Region) (ABIN570867)

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL1R2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-IL1R2 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: IL1R2

Sequenz: QYNITRSIEL RIKK

Spezifität: This antibody is expected to cross-react with both reported isoforms (NP_004624.1, NP_001248348.1). Reported variants represent identical protein: NP_775465.1, NP_004624.1

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QYNITRSIELRIKK

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 45 kDa band observed in lysates of cell lines LNCaP and MOLT-4 (calculated MW o 45.4 kDa according to NP_004624.1). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentrat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu IL1R2

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Andere Bezeichnung: IL1R2

Hintergrund: IL1R2, interleukin 1 receptor, type II, CD121b, IL1RB, MGC47725, antigen CDw121b, type II interleukin-1 receptor, beta

Gen-ID: 7850

NCBI Accession: NP_004624, NP_001248348

Pathways: NF-kappaB Signalweg