PRKAA2 Antikörper (Internal Region)

Produktnummer ABIN499148

Der Ziege Polyklonal anti-PRKAA2 Antikörper wird verwendet zum Nachweis von PRKAA2 in Proben von Human, Maus, Ratte, Rind (Kuh), Schwein und Hund. Er wurde validiert für WB und EIA.

Kurzübersicht für PRKAA2 Antikörper (Internal Region) (ABIN499148)

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PRKAA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-PRKAA2 Antikörper

Bindungsspezifität: Internal Region

Sequenz: CPLDALNTTK P

Spezifität: Recognizes AMPK2/PRKAA2.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006243.2. Genename: PRKAA2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL. This antibody detects a band of approx. 65 kDa in Human Heart and Liverlysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PRKAA2

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Andere Bezeichnung: PRKAA2

Hintergrund: AMPK alpha-2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK alpha-2 chain, AMPK2

Molekulargewicht: 62.3kDa (NP_006243.2).

Gen-ID: 5563

NCBI Accession: NP_006243

UniProt: P54646

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effekt