STAT4 Antikörper (Internal Region)

Produktnummer ABIN499125

Der Ziege Polyklonal anti-STAT4 Antikörper (ABIN499125) detektiert spezifisch STAT4 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für STAT4 Antikörper (Internal Region) (ABIN499125)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser STAT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-STAT4 Antikörper

Bindungsspezifität: AA 369-383, Internal Region

Sequenz: TLSNRRFVLC GTNVK

Spezifität: Recognizes STAT4 (aa 369-383).

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region (aa 369-383) of the protein sequence according to Human STAT4 (NP_003142.1). Genename: STAT4

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/1000. Western blot: 0.5-1.0 μg/mL. This antibody detects a band of approx. 85 kDa in transfected HEK293transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged). No bands were observed in mock-transfected HEK293 and the same band was observedusing anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Andere Bezeichnung: STAT4

Hintergrund: The STAT 4 protein is a transcription factor activated by hormone and cytokines. STAT 4 is most similar to STAT 1 (52 % ) and STAT 3 (47 %). Functionally STAT 4 is similar to other STAT family members in that it can be tyrosine phosphorylated by JAK 1 or JAK 2. STAT 4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT 4 can bind the interferon g activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. STAT 4 expression is restricted to the thymus, spleen, and testis.Synonyms: Signal transducer and activator of transcription 4

Gen-ID: 6775

NCBI Accession: NP_003142

UniProt: Q14765

Pathways: JAK-STAT Signalweg