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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) Antikörper

Antigen

zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)

Synonyme SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70
Epitope
Alternativen

pTyr493

Klonalität Polyklonal
Wirt
Alternativen

Kaninchen

Reaktivität
Alternativen

Human

Applikation
Alternativen Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
Produktnummer ABIN498719
Menge 100 µg  (1,0 mg/ml)  (Varianten)
Preis 320,00 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 7 bis 10 Werktagen

Produktbeschreibung

Produktmerkmale Synonyms: ZAP-70, ZAP 70, SRK, 70 kDa zeta-associated protein, Syk-related tyrosine kinase
Weitere Bezeichnung ZAP70
Gen-ID 7535
Swiss-Prot P43403
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Beschreibung The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), whichis a multisubunit complex assembled from at least six different genes. The TCR subunitsinclude the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and azeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds tothe phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCRzeta chain through two Src homology (SH2) domains. This binding results in thephosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylationon Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.
Spezifität P-ZAP-70 (pTyr493) pAb detects endogenous levels of p-ZAP-70 protein. Species: Human, Mouse, Rat. Others not tested.

Anwendungen

Applikationshinweise Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Konzentration 1,0 mg/ml
Reinigung Aff - Purified
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Lagerung Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen

Alternativen zu Antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", Typ "Antikörper" finden
Wirte Kaninchen (117), Maus (74)
Reaktivitäten Human (187), Maus (90), Ratte (Rattus) (65), Rind (Kuh) (18), Schwein (18), Hund (17), Pferd (16), Kaninchen (16), Huhn (15), Meerschweinchen (15)
Applikationen Western Blot (WB) (101), Durchflusszytometrie (FACS) (58), Immunfluoreszenz (IF) (34), Immunhistochemie (Paraffinschnitte) (IHC (p)) (34), Immunhistochemie (IHC) (33), ELISA (32), Immunpräzipitation (IP) (23), Enzyme Immunoassay (EIA) (21), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (9), Intrazelluläre Durchflusszytometrie (ICFC) (6), Immunzytochemie (ICC) (4), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
Konjugate FITC (11), PE (10), Biotin (9), Alkaline Phosphatase (AP) (4), HRP (3), PerCP,Cy5.5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
Epitope pTyr319 (10), pTyr493 (7), Ab-319 (5), Ab-493 (5), C-Term (5), pTyr292 (4), Center (3), AA 319-602 (1), Ab-292 (1), Ab-315 (1), pTyr315 (1), pTyr492 (1), pYTyr315/319 (1)