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Antikörper
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anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) Antikörper
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) Antikörper
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| Synonyme |
SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70 |
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Epitope
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pTyr319 (10), pTyr493 (7), Ab-319 (5), Ab-493 (5), C-Term (5), pTyr292 (4), Center (3), AA 319-602 (1), Ab-292 (1), Ab-315 (1), pTyr315 (1), pTyr492 (1), pYTyr315/319 (1)
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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Alternativen Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)
Western Blot (WB) (101), Durchflusszytometrie (FACS) (58), Immunfluoreszenz (IF) (34), Immunhistochemie (Paraffinschnitte) (IHC (p)) (34), Immunhistochemie (IHC) (33), ELISA (32), Immunpräzipitation (IP) (23), Enzyme Immunoassay (EIA) (21), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (9), Intrazelluläre Durchflusszytometrie (ICFC) (6), Immunzytochemie (ICC) (4), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN498719 |
| Menge |
100 µg (1,0 mg/ml) (Varianten) |
| Preis |
320,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: ZAP-70, ZAP 70, SRK, 70 kDa zeta-associated protein, Syk-related tyrosine kinase
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Weitere Bezeichnung
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ZAP70
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Gen-ID
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7535
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Swiss-Prot
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P43403
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Kreuzreaktivität
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Human, Maus, Ratte (Rattus)
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Beschreibung
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The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), whichis a multisubunit complex assembled from at least six different genes. The TCR subunitsinclude the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and azeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds tothe phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCRzeta chain through two Src homology (SH2) domains. This binding results in thephosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylationon Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.
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Spezifität
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P-ZAP-70 (pTyr493) pAb detects endogenous levels of p-ZAP-70 protein. Species: Human, Mouse, Rat. Others not tested.
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Applikationshinweise
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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1,0 mg/ml
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Reinigung
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Aff - Purified
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
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Lagerung
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", Typ "Antikörper" finden
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Wirte
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Kaninchen (117), Maus (74)
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Reaktivitäten
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Human (187), Maus (90), Ratte (Rattus) (65), Rind (Kuh) (18), Schwein (18), Hund (17), Pferd (16), Kaninchen (16), Huhn (15), Meerschweinchen (15)
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Applikationen
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Western Blot (WB) (101), Durchflusszytometrie (FACS) (58), Immunfluoreszenz (IF) (34), Immunhistochemie (Paraffinschnitte) (IHC (p)) (34), Immunhistochemie (IHC) (33), ELISA (32), Immunpräzipitation (IP) (23), Enzyme Immunoassay (EIA) (21), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (9), Intrazelluläre Durchflusszytometrie (ICFC) (6), Immunzytochemie (ICC) (4), Dot Blot (Dot) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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FITC (11), PE (10), Biotin (9), Alkaline Phosphatase (AP) (4), HRP (3), PerCP,Cy5.5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
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Epitope
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pTyr319 (10), pTyr493 (7), Ab-319 (5), Ab-493 (5), C-Term (5), pTyr292 (4), Center (3), AA 319-602 (1), Ab-292 (1), Ab-315 (1), pTyr315 (1), pTyr492 (1), pYTyr315/319 (1)
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